生物系
伊斯梅尔·本·福法纳
Assistant Professor of the Practice
伊斯梅尔·本·福法纳
Ph.D.
联系
希金斯414号厅
电话:617-552-3556
电子邮件: ismael.fofana@uc1112.com
ORCID 0000-0001-6384-2836
教育
- Ph.D.他是科迪大学的教授
太阳城官网兴趣
艾滋病毒/艾滋病疫苗, 艾滋病SIV/猕猴模型, Phage Display and Antibody 工程, Virus evolution and escape of antibody responses
部门
太阳城官网
HIV infection in humans and SIV infection in macaques are accompanied by the development of antibody responses to viral antigens, especially the envelope glycoprotein. 在大多数情况下, these antibodies fail to protect and infected subjects eventually progress to AIDS. Similarly, induction of protective antibodies by vaccination remains a daunting enterprise. Antibodies produced in response to viral infection can be classified into neutralizing and non-neutralizing. Neutralizing antibodies are highly desirable but have yet to be achieved by vaccination. 此外, a number of studies have now suggested a role for non-neutralizing antibodies in preventing infection and/or disease progression. 无论哪种情况, it often happens that resistant virus strains emerge in infected individuals prior to development of protective antibodies, rendering their presence of no benefit in preventing disease progression. Conventional methods for viral escape variant identification by Sanger sequencing and antibody immune response assessment by ELISA, EliSpot or viral neutralization assay appear insufficient to elucidate the mechanism of protection during the continuous race for survival between virus and host immune responses.
In order to study the interplay between virus and antibody, we use the SIV/macaque model of HIV/AIDS. We assess immune response to vaccination or infection by conventional parameters, 比如RNA病毒载量, 抗体效价(ELISA), 病毒中和试验, B细胞elisa试剂盒, B cell dysfunction/phenotypes (by multiparameter flow cytometry). We also use high throughput next generation sequencing to track the appearance of virus escape variants and to understand the evolution of immunoglobulin genes (IgG). 最后, we use phage display technology to select viral-specific monoclonal antibodies and genetic engineering to improve neutralizing potency of promising clones.
选定的出版物
- Seung yu Han, Alesia安东尼, 大卫•霍华德, 科比常, 张宇成, 马修Slein, Gintaras Deikus, 索非亚Kossida, 帕特里斯Duroux, marie - paule Lefranc, 罗伯特·P. 塞布拉，梅丽莎·L. 史密斯和 伊斯梅尔·本·F. Fofana (2018). 单分子偶联, Long Read Sequencing with IMGT/HighV-QUEST Analysis Expedites Identification of SIV gp140-Specific Antibodies from scFv Phage Display 库. 前面. Immunol., fimmu.2018.0032.
- Sergio Ita, Mayara R. Agostinho, Katherine Sullivan, Seung yu Han, Rana Akleh, Welkin E. 约翰逊和 伊斯梅尔·本·F. Fofana (2017). Analysis of SIVmac Envelope-specific antibodies selected via phage display. AIDS 太阳城官网 and Human Retroviruses. 33(8):869-879.
- Fofana IBF, 广告Colantonio, RK李维斯, 马Connole, JM Gillis, LR大厅, 年代佐藤, DT埃文斯, RP Johnson和WE Johnson. 2011. Flow-cytometry Based Identification of SIV Env-specific B Lymphocytes. J免疫方法. 370: 75-85
- Jia B, Serra-Moreno R, Neidemyer W, Jr.， Rahmberg A, Mackey J， Fofana IB, Johnson W, Westmoreland S, Evans D. 2009. Species-specific Activity of SIV Nef and HIV-1 Vpu in Overcoming Restriction by Tetherin/BST2. 公共科学图书馆Pathog. 2009 May; 5(5):e1000429.
- McNatt MW, Zang T, Hatziioannou T, Bartlett M, Fofana IB, Johnson WE, Neil SJD and Bieniasz P 2009. Species-specific activity of HIV-1 Vpu and positive selection of tetherin transmembrane domain variants. 公共科学图书馆Pathog. 2009 Feb; 5 (2), e1000300.
- Okada H，张欣， Fofana IB, Nagai M, Suzuki H, Ohashi T, and Shida H (2009). Synergistic effect of human-CycT1 and -CRM1 on HIV-1 propagation in rat T cells and macrophages. Retrovirology, 6:43.
- Suzuki H, Kidokoro M， Fofana IB, Ohashi T, Okamura T, Matsuo K, Yamamoto N, Shida H (2008). Immunogenicity of newly constructed attenuated vaccinia strain LC16m8∆ that expresses SIV Gag protein. 疫苗, 27(7): 966-71.
- 恩登古鲁，J，莱格，J.P., Fofana,我.B.F.， Aveling, T.A.S，汤普森，G. 福奎特，C.M. (2006).). Identification of a defective molecule derived from DNA-A of the bipartite begomovirus of East African cassava mosaic virus. 植物病理学. 55, 2-10
- Fofana IBF, Sangaré A, Collier R, Taylor C and Fauquet C (2004). A geminivirus-Induced gene silencing system for gene function validation in cassava. 分子生物学, 56: 613–624
- I.B.F Fofana, A. Sangare J. Ndunguru K. 卡恩和C.M. 法奎特,(2003). Principle of control of virus diseases in developing countries. In: Virus and Virus-like Diseases of Major Crops in Developing Countries. Gad Loebenstein and George Thottappilly (eds). Kluwer学术出版社. pp 31-54
- Kouassi, IBF Fofana, [J] .科学通报，2000. Collection and Preservation of Mushrooms genetic resources in Ivory Coast, for their utilization for food and Agriculture. In: Mushroom Genetic 资源 for Food and Agriculture. labarre和Menini主编.). 全球 Network on Mushrooms under the aegis of F.A.O. pp 173-185